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    The Identification of Eggs of Echinococcus by Immunofluorescence Using a Specific Anti-oncospheral Monoclonal antibody

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    Research article (2.106Mb)
    Publication Date
    1/1/1986
    Authors
    Craig, P.S.
    Macpherson, C.N.
    Nelson, G.S .
    Type
    Article, Journal
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    Citation

    Craig PS, Macpherson CN, Nelson GS. The identification of eggs of Echinococcus by immunofluorescence using a specific anti-oncospheral monoclonal antibody. The American Journal of Tropical Medicine and Hygiene. 1986 Jan;35(1):152-158. DOI: 10.4269/ajtmh.1986.35.152. PMID: 3511745.

    Abstract/Overview

    A relatively simple and specific test has been developed to distinguish eggs of Echinococcus from those of other morphologically identical taeniid species. A specific anti-Echinococcus oncosphere monoclonal antibody was produced which binds in an indirect immunofluorescence test to egg-derived oncospheres of E. granulosus but not to those of other taeniid species, such as Taenia hydatigena, T. saginata, T. pisiformis, T. ovis, T. multiceps, or T. taeniaeformis. Specific fluorescence was obtained with oncospheres of E. granulosus derived from either hatch/activated viable eggs using artificial intestinal fluid or from hypochlorite/detergent treated eggs. The potential use of this test in the study of the transmission of Echinococcus in Turkana, Kenya, is discussed.

    Subject/Keywords
    Test; Eggs; Immunofluorescence test; Turkana; Kenya
    Publisher
    The American journal of tropical medicine and hygiene
    ISSN
    0020-7519; PMID: 3511745
    Permalink
    http://repository.amref.org/handle/123456789/218
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    • General - GEN [355]

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